Principle of the Procedure

Total bilirubin (TBil) is the sum of direct bilirubin and indirect bilirubin. The determination of serum total bilirubin is an important test item in liver and gallbladder function test. It can accurately reflect the degree of jaundice and has important significance for clinical diagnosis of recessive jaundice. This Kit provides a convenient tool for detection of TBIL. The principle is that in the presence of surfactant, total bilirubin can be oxidized by sodium nitrite to produce biliverdin. The content of total bilirubin can be calculated by detecting the change of wavelength at 450nm.