Principle of the Procedure

Uric Acid (UA) Assay Kit provides a simple method for detecting Uric Acid (UA) concentration in a variety of biological samples such as Animal Tissues, Serum, Plasma, Urine and other biological Fluids. In the assay, Uricase can catalyze UA to produce allantoin, CO2 and H2O2; H2O2 oxidizes Fe2+ in potassium ferrocyanide to produce Fe3+; Fe3+ further condenses with phenol and 4-aminoantipyrine to form red quinone compounds which has a characteristic absorption peak at 505 nm. The UA content can be calculated by measuring the light absorption at this wavelength.