Principle of the Procedure

Blood urea nitrogen (BUN) is a blood test performed as a marker of kidney function. It is part of the basic or comprehensive metabolic panel done for health screening. It is also used to monitor the progression of kidney failure.In the assay,Sample of urea nitrogen in Iron(III) chloride-Phosphoric acid solution and Diacetyl monoxime and aminothiourea were boiled,produces a red diazine compounds which has a characteristic absorption peak at 540 nm. The Urea Nitrogen present in the sample is proportional to the signal obtained.