Principle of the Procedure

Vitamin E (VE) is a fat-soluble vitamin, also known as tocopherol or pregnanthogenic phenol. It is found in edible oils, fruits, vegetables and food, and is one of the most important antioxidants in organisms. It can prevent the damage of unsaturated fatty acids by peroxidation, maintain the integrity and normal function of the membrane of unsaturated fatty acids, delay aging and prevent hemolytic anemia, and has high application value in medicine, cosmetics, health products and food industries. This Kit provides a simple method for detecting Vitamin E concentration in a variety of biological samples such as Animal and Plant Tissues, Cells, Plasma, Serum or other biological fluids. The principle is that Fe3+ reduced by VE is Fe2+, and Fe2+ forms colored complexes with 1, 10-phenanthroline, which has characteristic absorption peak at 530nm. The absorbance at 530nm is directly proportional to Vitamin E concentration in the sample.