Quanticon™ TimeSaver Porcine ACAT2(Acetyl Coenzyme A Acetyltransferase 2) ELISA Kit
Quanticon™ TimeSaver Porcine ACAT2(Acetyl Coenzyme A Acetyltransferase 2) ELISA Kit
Quanticon™ TimeSaver Porcine ACAT2(Acetyl Coenzyme A Acetyltransferase 2) ELISA Kit
Principle of the Procedure
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine ACAT2. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Porcine ACAT2 are added to the micro ELISA plate wells. Porcine ACAT2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Porcine ACAT2. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Porcine ACAT2. You can calculate the concentration of Porcine ACAT2 in the samples by comparing the OD of the samples to the standard curve.
Product Name | Quanticon™ TimeSaver Porcine ACAT2(Acetyl Coenzyme A Acetyltransferase 2) ELISA Kit | |
SKU | ABQ-FS96-ACAT2pr | |
Applications | ELISA | |
Series of Elisa | TimeSaver | |
Reactivity | Porcine | |
Alternative Name | ACTL, Acetoacetyl Coenzyme A Thiolase, Acetyl-CoA transferase-like protein, Cytosolic acetoacetyl-CoA thiolase | |
Standard | 5000pg/mL | |
Sensitivity | 34.77pg/mL | |
Detection Range | 78.13-5000pg/mL | |
Sample Type | Serum, Plasma, Cell culture supernatant and Other types of sample | |
Sample Volume | 50μL | |
Assay Type | Sandwich | |
Assay Duration | 1.5 H | |
Detection Wavelength | 450nm | |
Storage | 2-8oC/-20 oC | |
Kit components & Storage An unopened kit can be stored at 2-8℃ for 12 months. If the opened kit is not used up, store the items separately according to the following conditions. | ||
Item | Specifications | Storage |
Micro ELISA Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips 24T: 8 wells×3 strips |
-20℃, 12 months |
Reference Standard | 96T: 2 vials 48T: 1 vial 24T: 1 vial | |
Concentrated HRP Conjugate (100X) | 96T:1 vial, 60μL 48T:1 vial, 30μL 24T:1 vial, 30μL | |
Reference Standard & Sample Diluent | 1 vial, 20 mL |
2-8℃, 12 months |
HRP Conjugate Diluent | 1 vial, 13 mL | |
Concentrated Wash Buffer (25X) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃, 12 months |
Plate Sealer | 5 pieces |
RT |
Product Description | 1 copy | |
Certificate of Analysis | 1 copy | |
Citations