Quanticon™ TimeSaver Human ACO-2(Aconitase 2) ELISA Kit
Quanticon™ TimeSaver Human ACO-2(Aconitase 2) ELISA Kit
Quanticon™ TimeSaver Human ACO-2(Aconitase 2) ELISA Kit
Principle of the Procedure
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ACO-2. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Human ACO-2 are added to the micro ELISA plate wells. Human ACO-2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human ACO-2. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human ACO-2. You can calculate the concentration of Human ACO-2 in the samples by comparing the OD of the samples to the standard curve.
Product Name | Quanticon™ TimeSaver Human ACO-2(Aconitase 2) ELISA Kit | |
SKU | ABQ-FS96-ACO2hu | |
Applications | ELISA | |
Series of Elisa | TimeSaver | |
Reactivity | Human | |
Alternative Name | Aconitate hydratase, mitochondrial, Aconitase, Citrate hydro-lyase, ACO2 | |
Standard | 20ng/mL | |
Sensitivity | 0.1ng/mL | |
Detection Range | 0.31-20ng/mL | |
Sample Type | Serum, Plasma, Cell culture supernatant and Other types of sample | |
Sample Volume | 50μL | |
Assay Type | Sandwich | |
Assay Duration | 1.5 H | |
Detection Wavelength | 450nm | |
Storage | 2-8oC/-20 oC | |
Kit components & Storage An unopened kit can be stored at 2-8℃ for 12 months. If the opened kit is not used up, store the items separately according to the following conditions. | ||
Item | Specifications | Storage |
Micro ELISA Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips 24T: 8 wells×3 strips |
-20℃, 12 months |
Reference Standard | 96T: 2 vials 48T: 1 vial 24T: 1 vial | |
Concentrated HRP Conjugate (100X) | 96T:1 vial, 60μL 48T:1 vial, 30μL 24T:1 vial, 30μL | |
Reference Standard & Sample Diluent | 1 vial, 20 mL |
2-8℃, 12 months |
HRP Conjugate Diluent | 1 vial, 13 mL | |
Concentrated Wash Buffer (25X) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃, 12 months |
Plate Sealer | 5 pieces |
RT |
Product Description | 1 copy | |
Certificate of Analysis | 1 copy | |
Citations