Quanticon™ TimeSaver Porcine ACP3/PAP(Prostatic Acid Phosphatase) ELISA Kit
Quanticon™ TimeSaver Porcine ACP3/PAP(Prostatic Acid Phosphatase) ELISA Kit
Quanticon™ TimeSaver Porcine ACP3/PAP(Prostatic Acid Phosphatase) ELISA Kit
Principle of the Procedure
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine ACP3/PAP. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Porcine ACP3/PAP are added to the micro ELISA plate wells. Porcine ACP3/PAP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Porcine ACP3/PAP. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Porcine ACP3/PAP. You can calculate the concentration of Porcine ACP3/PAP in the samples by comparing the OD of the samples to the standard curve.
Product Name | Quanticon™ TimeSaver Porcine ACP3/PAP(Prostatic Acid Phosphatase) ELISA Kit | |
SKU | ABQ-FS96-ACP3pr | |
Applications | ELISA | |
Series of Elisa | TimeSaver | |
Reactivity | Porcine | |
Alternative Name | PSAP, ACPP, PAP, PAPf39, 5′-NT, Prostatic Specific Acid Phosphatase, Ecto-5′-Nucleotidase | |
Standard | 40ng/mL | |
Sensitivity | 0.24ng/mL | |
Detection Range | 0.63-40ng/mL | |
Sample Type | Serum, Plasma, Cell culture supernatant and Other types of sample | |
Sample Volume | 50μL | |
Assay Type | Sandwich | |
Assay Duration | 1.5 H | |
Detection Wavelength | 450nm | |
Storage | 2-8oC/-20 oC | |
Kit components & Storage An unopened kit can be stored at 2-8℃ for 12 months. If the opened kit is not used up, store the items separately according to the following conditions. | ||
Item | Specifications | Storage |
Micro ELISA Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips 24T: 8 wells×3 strips |
-20℃, 12 months |
Reference Standard | 96T: 2 vials 48T: 1 vial 24T: 1 vial | |
Concentrated HRP Conjugate (100X) | 96T:1 vial, 60μL 48T:1 vial, 30μL 24T:1 vial, 30μL | |
Reference Standard & Sample Diluent | 1 vial, 20 mL |
2-8℃, 12 months |
HRP Conjugate Diluent | 1 vial, 13 mL | |
Concentrated Wash Buffer (25X) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃, 12 months |
Plate Sealer | 5 pieces |
RT |
Product Description | 1 copy | |
Certificate of Analysis | 1 copy | |
Citations