Product Name

CheKine™ Micro Proline (PRO) Assay Kit

Catalogue No

KTB1430

Applications Notes

The kit provides a simple method for detecting Pro content in a variety of biological samples such as serum, plasma, Plant or animal tissue, cells, and bacteria. In the assay, Pro is extracted with sulfosalicylic acid (SA). After heat treatment, Pro reacts with acidic ninhydrin solution to produce red; after extraction with toluene, the absorbance is measured at 520 nm.

Kit components

• Extraction Buffer

• Assay Buffer I

•Chromogen

•Pro Substrate

Features & Benefits 

• Determination of Pro content in a variety of biological samples such as serum, plasma, Plant or animal tissue, cells, and bacteria.

• A broad range linearity: 0.5-80 μg/mL.

• Detailed sample preparation and result calculation methods are provided.

• Simple operation steps.

• Standard and standard curve line are provided.

Storage

Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.

Shipping

Gel pack with blue ice.

Formulations

Liquid solution ready to use

Background

Pro is widely present in animals, plants, microorganisms and cultured cells. Under adversity conditions, the Pro content in plants increased significantly. The increased amount of Pro reflects stress resistance to a certain extent, and varieties with strong drought resistance tend to accumulate more proline. Therefore, the increased amount of proline can be used as one of the physiological indicators of stress resistance breeding.