This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Rat IFN-γ. Standards or samples containing Rat IFN-γ are added to the plate and reacted with capture antibody. A second anti-Rat IFN-γ antibody labeled biotin is then added and binds to Rat IFNγ captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Rat IFN-γ-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Rat IFN-γ in the samples is then calculated from the OD value by establishing a standard curve.
Cat no:
ABQ-ES96-IFNΓrt
ABQ-ES96-IFNΓrt
Size:
Rate
Rate
96T:
$450
$450
48T:
$230
$230
Datasheet
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MS/DS
COA
| Product name | Quanticon™ Rat IFN-γ(Interferon Gamma) ELISA Kit |
| SKU | ABQ-ES96-IFNΓrt |
| Applications | ELISA |
| Reactivity | RAT |
| Sensitivity | 10 pg/mL. |
| Detection Range | 31.25-2500pg/mL. |
| Sample Type | Serum, Plasma, Tissue homogenate, Cell lysates and Cell culture supernatant and other biological fluids |
| Sample Volume | 100 μL |
| Assay Type | Sandwich |
| Assay Duration | 3.5H |
| Detection Wavelength | 450nm |
| Storage | 2-8oC/-20 oC |
Citations
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo.
| Product name | Quanticon™ Rat IFN-γ(Interferon Gamma) ELISA Kit |
| SKU | ABQ-ES96-IFNΓrt |
| Applications | ELISA |
| Reactivity | RAT |
| Sensitivity | 10 pg/mL. |
| Detection Range | 31.25-2500pg/mL. |
| Sample Type | Serum, Plasma, Tissue homogenate, Cell lysates and Cell culture supernatant and other biological fluids |
| Sample Volume | 100 μL |
| Assay Type | Sandwich |
| Assay Duration | 3.5H |
| Detection Wavelength | 450nm |
| Storage | 2-8oC/-20 oC |
Citations
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo.
