Quanticon™ 17-OHP(17-Hydroxyprogesterone) ELISA Kit
Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 17-OHP. During the reaction, 17-OHP in the sample or standard competes with a fixed amount of 17-OHP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 17-OHP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 17-OHP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Product Name | Quanticon™ 17-OHP(17-Hydroxyprogesterone) ELISA Kit |
SKU | ABQ-EC96-17OHPge |
Applications | ELISA |
Reactivity | General |
Alternative Name | 17-OHP, 17-Hydroxyprogesterone |
Standard | 2000pg/mL. |
Sensitivity | 87.3 pg/mL. |
Detection Range | 312.5-20000 pg/mL |
Sample Type | Serum, Plasma, Tissue homogenate and Other biological samples |
Sample Volume | 50 ÎĽL |
Assay Type | Competitive |
Assay Duration | 1.5H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
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