Principle of the Procedure

Glutamic-oxalacetic Transaminase(GOT)/Aspartate Aminotransferase(AST) is widely present in animals, plants, microorganisms and cultured cells. It catalyzes the reversible amino reaction and is an important enzyme for amino acid metabolism. Serum GOT/AST activity level is an important biochemical indicator of liver damage, which can reflect the severity of liver damage. In addition, GOT/AST has the highest content in cardiomyocytes and is generally used as an auxiliary examination for myocardial infarction and myocarditis in clinical practice. This kit provides a simple method for detecting GOT/AST activity in a variety of biological samples such as Animal Tissues, Plant Tissues, Cell culture (Adherent or Suspension Cells), Serum, Bacteria. In the assay, Glutamic-oxalacetic Transaminase/Aspartate Aminotransferase (GOT/AST) catalyzes the transamination reaction of α-ketoglutarate and L-aspartic acid at 37℃ and pH7.4 to produce Glutamic Acid and Pyruvate; Pyruvate can interact with 2,4- Dinitrophenylhydrazine to produce Pyruvate Phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of Pyruvate Phenylhydrazone increase at 505 nm can reflect GOT/AST activity.