Principle of the Procedure

Free Fatty Acids (FFA), also known as non-esterified fatty acids, are not only the product of fat hydrolysis, but also the substrate of fat synthesis, circulating in plasma combined with albumin. The concentration of FFA in serum is related to lipid metabolism, carbohydrate metabolism and endocrine function. The concentration of FFA will increase due to diabetes, severe liver dysfunction, hyperthyroidism and other diseases. This Kit provides a convenient tool for detection of FFA. The principle is that the FFA combines with copper ions to form copper salt of fatty acid, which is soluble in chloroform. The content of free fatty acid can be calculated by determining the content of copper ion with copper reagent method.