Principle of the Procedure

Glutathione Reductases (GR) is a flavin protein redox enzyme widely present in eukaryotes and prokaryotes. GR catalyzes the reduction of GSSG to GSH. It is one of the key enzymes in the glutathione redox cycle (generally GR is replaced by Thioredoxin Reductase (TrxR) in insects). GR catalyzes the reduction of GSSG by NADPH to GSH, which helps maintain the GSH/GSSG ratio in the body. GR plays a key role in scavenging reactive oxygen species in response to oxidative stress, and GR is also involved in the ascorbate-glutathione cycle pathway. Glutathione Reductases (GR) Assay Kit provides a simple method for detecting GR activity in a variety of biological samples, such as Serum, Plasma, Animal and Plant Tissues, Cells, Bacteria. GR can catalyze the reduction of NADPH to GSSG to regenerate GSH, and NADPH dehydrogenates to produce NADP+; NADPH has a characteristic absorption peak at 340 nm, while NADP+ has no absorption peak at this wavelength; NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GR activity.