Principle of the Procedure

High Density Lipoprotein Cholesterol (HDL-C) as one of the serum proteins, mainly synthesized by the liver. It’s carrying cholesterol from surrounding tissues, which is then converted into bile acids or directly excreted from the intestine through bile. It is an anti-atherosclerotic plasma lipoprotein, a protective factor for coronary heart disease (CHD), commonly known as “vascular scavenger”, and is an important reference index for the clinical diagnosis of coronary heart disease (CHD). High Density Lipoprotein Cholesterol (HDL-C) Assay Kit provides a convenient tool for detection of HDL-C. The principle is that HDL-C in serum was separated with precipitant, and cholesterol esterase was used to catalyze the hydrolysis of cholesterol ester into free cholesterol (FC) and free fatty acid (FFA), so as to convert cholesterol ester into FC. Furthermore, cholesterol oxidase catalyzed FC oxidation to produce △4-cholestenone and H2O2. Then peroxidase catalyzes the oxidation of 4-amino-antipyrine and phenol with H2O2 to produce red quinone compounds has a characteristic absorption peak at 500nm.