Principle of the Procedure

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse PG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse PG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse PG in the samples is then determined by comparing the OD of the samples to the standard curve.

Principle of the Procedure

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PGH2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGH2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGH2 in the samples is then determined by comparing the OD of the samples to the standard curve.