Quanticon Human ADP/Acrp30(Adiponectin) ELISA Kit

Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADP/Acrp30. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ADP/Acrp30. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADP/Acrp30, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ADP/Acrp30 in the samples is then determined by comparing the OD of the samples to the standard curve.

Specification

PRECISION

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter- Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess Inter-Assay precision.

CV (%)= SD/mean x 100

Intra-Assay: CV<5%

Inter- Assay: CV<8%