Please, enable Compare.
Please, enable Wishlist.

No products in the cart.

Principle

This assay employs the quantitative sandwich enzyme immunoassay technique. The micro plate provided in this kit has been pre-coated with an antibody specific to Human ASGR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ASGR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ASGR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm±3nm. The concentration of ASGR1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Specification

Product name Quanticon Human ASGR1(Asialoglycoprotein Receptor 1) ELISA Kit
Cat no ABQ-ES96-ASGR1hu
Applications ELISA
Reactivity Human
Alternative Name ASGR1
Standard 10ng/mL
Sensitivity 0.1 ng/mL
Detection Range 0.16-10ng/mL
Sample Type Serum, plasma and Cell culture supernatant
Sample Volume 100 μL
Assay Type Sandwich
Assay Duration 3.5H
Detection Wavelength 450nm
Storage 2-8oC

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid-range and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid-range and high level were tested on 3 different plates, 20 replicates in each plate, respectively

Intra-Assay: CV<5%

Inter-Assay: CV<8%

Quanticon Human ASGR1(Asialogl...

You may add any content here from XStore Control Panel->Sales booster->Request a quote->Ask a question notification

At sem a enim eu vulputate nullam convallis Iaculis vitae odio faucibus adipiscing urna.

Ask an expert