Quanticon™ Human CLIP(Corticotropin Like Intermediate Lobe Peptide) ELISA Kit
Quanticon™ Human CLIP(Corticotropin Like Intermediate Lobe Peptide) ELISA Kit
Quanticon™ Human CLIP(Corticotropin Like Intermediate Lobe Peptide) ELISA Kit
Principle of the Procedure
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CLIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CLIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CLIP in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name | Quanticon™ Human CLIP(Corticotropin Like Intermediate Lobe Peptide) ELISA Kit |
SKU | ABQK-EC96-CLIPhu |
Applications | ELISA |
Reactivity | Human |
Alternative Name | CLIP |
Standard | 1000 pg/mL |
Sensitivity | 4.94 pg/mL |
Detection Range | 15.63-1000 pg/mL |
Sample Type | Serum, Plasma, Tissue homogenate, Urine, Saliva, Cell culture supernatant and Other biological samples |
Sample Volume | 50 ÎĽL |
Assay Type | Competitive Inhibition |
Assay Duration | 1.5 H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
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