Quanticon Human hs-CRP(high-sensitivity C-Reactive Protein) ELISA Kit

Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CAT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CAT, biotin-conjugated antibody and enzymeconjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CAT in the samples is then determined by comparing the OD of the samples to the standard curve..

Specification

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Intra-Assay: CV<5%

Inter-Assay: CV<8%