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Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGF1R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IGF1R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IGF1R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IGF1R in the samples is then determined by comparing the OD of the samples to the standard curve.

Specification

Product name Quanticon Human IGF1R(Insulin Like Growth Factor 1 Receptor) ELISA Kit
SKU ABQ-ES96-IGF1Rhu
Applications ELISA
Reactivity Human
Alternative Name CD221, IGFIR, IGFR, JTK13
Standard 20ng/mL
Sensitivity 0.19 ng/mL
Detection Range 0.32-20ng/mL
Sample Type Tissue homogenate and Cell lysate
Sample Volume 100 μL
Assay Type Sandwich
Assay Duration 3.5H
Detection Wavelength 450nm
Storage 2-8oC/-20 oC

PRECISION

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8

Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 87-99 93
EDTA plasma (n=8) 80-95 86
Cell culture media (n=8) 95-107 101
Quanticon Human IGF1R(Insulin ...

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