Quanticon Human LEP(Leptin) ELISA Kit

Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LEP in the samples is then determined by comparing the OD of the samples to the standard curve.

Specification

PRECISION

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter- Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess Inter-Assay precision.

CV (%)= SD/mean x 100

Intra-Assay: CV<5%

Inter- Assay: CV<8%