Quanticon Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit

Principle

This assay employs the quantitative sandwich enzyme immunoassay technique. The micro plate provided in this kit has been pre-coated with an antibody specific to Human NGAL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NGAL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NGAL, biotinconjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm±3nm. The concentration of NGAL in the samples is then determined by comparing the OD of the samples to the standard curve.

Specification

PRECISION

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate,respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Intra-Assay: CV<5%

Inter-Assay: CV<8%