Principle of the Procedure

Lactate is an important intermediate product in the metabolic process of organisms, which is closely related to glucose metabolism, lipid metabolism, protein metabolism and intracellular energy metabolism. The content of lactate is an important indicator to evaluate glycogen metabolism and aerobic metabolism. Abnormally high levels of lactate have been linked to pathological conditions such as cancer, diabetes and lactic acidosis.The Kit provides a convenient means for detecting L(+)-Lactate in biological samples such as animal and plant tissues, cells, serum, plasma or other liquid samples. In this kit, lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a tetrazolium salt WST-8 dye to form a colorimetric (450 nm) product, proportional to the lactate present.