Principle of the Procedure

Oxygen free radicals react on lipid unsaturated fatty acids to produce lipid peroxidation. The latter gradually breaks down into a series of complex compounds, including malondialdehyde (MDA). Lipid oxidation levels can be measured by detecting MDA levels. Lipid peroxidation may contribute to many diseases, including atherosclerosis, diabetes, and Alzheimer’s disease. Malondialdehyde (MDA) Assay kit provides a convenient tool for detection of the MDA present in a variety of samples. MDA can be condensed with thiobarbituric acid (TBA) under acidic and high temperature conditions to produce the brown-red trimethyltran (3,5, 5-trimethyloxazol-2, 4-dione) with a maximum absorption wavelength of 532 nm. Meanwhile, the absorbance at 600 nm was measured to eliminate the interference of sucrose, and MDA was calculated by the difference between the absorbance at 532 nm and 600 nm.