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Principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IAPP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IAPP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IAPP in the samples is then determined by comparing the OD of the samples to the standard curve.

Specification

Product name Quanticon Mouse IAPP(Islet Amyloid Polypeptide) ELISA Kit
SKU ABQ-EC96-IAPPms
Applications ELISA
Reactivity Mouse
Alternative Name DAP, IAP, Amylin
Standard 4000pg/mL
Sensitivity 37.5 pg/mL
Detection Range 62.5-4000pg/mL
Sample Type Serum, Plasma, Tissue homogenate and Other biological samples
Sample Volume 50 μL
Assay Type Competitive
Assay Duration 1.5H
Detection Wavelength 450nm
Storage 2-8oC/-20 oC

PRECISION

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8

Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range(%) Average Recovery(%)
Serum (n=8) 82-95 88
EDTA plasma (n=8) 95-107 101
Cell culture media (n=8) 80-95 86
Quanticon Mouse IAPP(Islet Amy...

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