Quanticon™ Rat HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit
Principle of the Procedure
This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Rat HIF-1α. Standards or samples containing Rat HIF-1α are added to the plate and reacted with capture antibody. A second anti-Rat HIF-1α antibody labeled biotin is then added and binds to Rat HIF-1α captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Rat HIF-1α-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Rat HIF-1α in the samples is then calculated from the OD value by establishing a standard curve.
Product Name | Quanticon™ Rat HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit |
SKU | ABQ-ES96-HIF1Art |
Applications | ELISA |
Mouse | Rat |
Alternative Name | HIF1A, HIF-1A, MOP1, PASD8, bHLHe78 |
Standard | 500pg/mL. |
Sensitivity | 4.69 pg/mL. |
Detection Range | 7.82-500pg/mL. |
Sample Type | Serum, Plasma, Tissue homogenate and Other biological samples |
Sample Volume | 100 μL |
Assay Type | Sandwich |
Assay Duration | 3.5 H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
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