Quanticon™ Rat IFN-γ(Interferon Gamma) ELISA Kit

This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Rat IFN-γ. Standards or samples containing Rat IFN-γ are added to the plate and reacted with capture antibody. A second anti-Rat IFN-γ antibody labeled biotin is then added and binds to Rat IFNγ captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Rat IFN-γ-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Rat IFN-γ in the samples is then calculated from the OD value by establishing a standard curve.

Cat no: ABQ-ES96-IFNΓrt
ABQ-ES96-IFNΓrt
Size: Rate
Rate
96T: $450
$450
48T: $230
$230

Product name

Quanticon™ Rat IFN-γ(Interferon Gamma) ELISA Kit

SKU

ABQ-ES96-IFNΓrt

Applications

ELISA

Reactivity

RAT

Sensitivity

10 pg/mL.

Detection Range

31.25-2500pg/mL.

Sample Type

Serum, Plasma, Tissue homogenate, Cell lysates and Cell culture supernatant and other biological fluids

Sample Volume

100 μL

Assay Type

Sandwich

Assay Duration

3.5H

Detection Wavelength

450nm

Storage

2-8oC/-20 oC

 

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