Quanticon™ Rat IL-33(Interleukin 33) ELISA Kit
This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been precoated with an affinity purified antibody to Rat IL-33. Standards or samples containing Rat IL-33 are added to the plate and reacted with capture antibody. A second anti-Rat IL-33 antibody labeled biotin is then added and binds to Rat IL-33 captured on the plate. After that, Streptavidin-Horseradish Peroxidase (SA-HRP) is added to form a sandwich complex of solid phase antibody-Rat IL-33-biotin labeled antibody-SA-HRP.And then, TMB substrate solution is added to all wells and incubated. An enzymecatalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Rat IL-33 in the samples is then calculated from the OD value by establishing a standard curve.
Product name | Quanticon™ Rat IL-33(Interleukin 33) ELISA Kit |
SKU | ABQ-ES96-IL33rt |
Applications | ELISA |
Reactivity | RAT |
Sensitivity | 1.0 pg/mL. |
Detection Range | 50-1000pg/mL. |
Sample Type | Serum, Plasma, Tissue homogenate, Cell lysates and Cell culture supernatant and other biological fluids |
Sample Volume | 100 ÎĽL |
Assay Type | Sandwich |
Assay Duration | 3.5H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
Citations
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