Quanticon™ Rat INS(Insulin) ELISA Kit
Principle of the Procedure
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat INS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat INS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat INS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat INS in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name | Quanticon™ Rat INS(Insulin) ELISA Kit |
SKU | ABQK-ES96-INSrt |
Applications | ELISA |
Reactivity | Rat |
Alternative Name | Insulin |
Standard | 10 ng/mL |
Sensitivity | 0.052 ng/mL |
Detection Range | 0.16-10 ng/mL |
Sample Type | Serum, Plasma, Tissue homogenate, Urine, Saliva, Feces, Cell culture supernatants and other biological fluids and Cerebrospinal fluid (CSF) |
Sample Volume | 100 ÎĽL |
Assay Type | Sandwich |
Assay Duration | 3.5 H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
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