Principle of the Procedure

Glutathione is a Tripeptide of Glycine, Glutamic acid and Cysteine. In the red blood cell, reduced Glutathione (GSH) is the key to maintain hemoglobin in reduced state and protect cells from Oxidative damage. GSH is the most important antioxidant sulfhydryl compound in cells, which plays an important role in oxidation resistance, protein sulfhydryl protection and amino acid transport across membrane. The ratio between reduced and oxidized Glutathione (GSH/GSSG) is the main indicator of the cell’s redox state. Therefore, measuring the content of GSH and GSSG and the ratio of GSH/GSSG in cells can reflect the redox state of cells.This kit provides a simple method for detecting the content of GSH in a variety of biological samples, such as Serum, Plasma, Animal and Plant Tissues, Red Blood cells, Cells, Bacteria. DTNB reacts with reduced Glutathione to form a yellow product. The optical density measured at 412 nm, can directly reflect Glutathione concentration in the sample.