Quanticon™ TimeSaver Human ACTH(Adrenocorticotropic Hormone) ELISA Kit
Quanticon™ TimeSaver Human ACTH(Adrenocorticotropic Hormone) ELISA Kit
Quanticon™ TimeSaver Human ACTH(Adrenocorticotropic Hormone) ELISA Kit
Principle of the Procedure
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human ACTH. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human ACTH are added to the micro ELISA plate wells. Human ACTH in samples (or standards) competes with a fixed amount of Human ACTH on the solid phase supporter for sites on the HRP linked detection antibody specific to Human ACTH. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human ACTH in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name | Quanticon™ TimeSaver Human ACTH(Adrenocorticotropic Hormone) ELISA Kit | |
SKU | ABQ-FS96-ACTHhu | |
Applications | ELISA | |
Series of Elisa | TimeSaver | |
Reactivity | Human | |
Alternative Name | ACT, ACT-G2, ACTA3, ACTE, ACTL3, ACTSG, Alpha-actin-3 | |
Standard | 1000pg/mL | |
Sensitivity | 7.95pg/mL | |
Detection Range | 15.63-1000pg/mL | |
Sample Type | Serum, Plasma, Cell culture supernatant and Other types of sample | |
Sample Volume | 50μL | |
Assay Type | Competitive | |
Assay Duration | 45min | |
Detection Wavelength | 450nm | |
Storage | 2-8oC/-20 oC | |
Kit components & Storage An unopened kit can be stored at 2-8℃ for 12 months. If the opened kit is not used up, store the items separately according to the following conditions. | ||
Item | Specifications | Storage |
Micro ELISA Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips 24T: 8 wells×3 strips |
-20℃, 12 months |
Reference Standard | 96T: 2 vials 48T: 1 vial 24T: 1 vial | |
Concentrated HRP Conjugate (100X) | 96T:1 vial, 60μL 48T:1 vial, 30μL 24T:1 vial, 30μL | |
Reference Standard & Sample Diluent | 1 vial, 20 mL |
2-8℃, 12 months |
HRP Conjugate Diluent | 1 vial, 13 mL | |
Concentrated Wash Buffer (25X) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃, 12 months |
Plate Sealer | 5 pieces |
RT |
Product Description | 1 copy | |
Certificate of Analysis | 1 copy | |
Citations