Quanticon™ XcelStep Rat ADP(Adiponectin) ELISA Kit
Principle of the Procedure
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADP, and the Rat ADP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat ADP . After TMB substrate solution is added, only those wells that contain Rat ADP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ADP in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name | Quanticon™ XcelStep Rat ADP(Adiponectin) ELISA Kit |
SKU | ABQK-ZS96-ADPrt |
Applications | ELISA |
Reactivity | Rat |
Alternative Name | ApM1, AdipoQ, Acrp30 |
Standard | 500 ng/mL |
Sensitivity | 4.75 ng/mL |
Detection Range | 7.82-500 ng/mL |
Sample Type | Serum, Plasma, Tissue homogenate, Urine, Saliva, Feces, Cell culture supernatants and other biological fluids and Cerebrospinal fluid (CSF) |
Sample Volume | 100 ÎĽL |
Assay Type | Sandwich |
Assay Duration | 3.5 H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
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