Tris-acetate-EDTA (TAE) Buffer is designed to be used in molecular biology for agarose gel electrophoresis to separate nucleic acids like DNA and RNA. It consists of Tris base , acetic acid , and EDTA ,functioning as both a running buffer and a gel preparation buffer. It maintains pH around 8.3 and protects DNA degradation during run. eliminates DNAse activity TAE is particularly suited for the separation of a broad range of DNA fragments, especially for preparative electrophoresis and cloning experiments.