Quanticon™ cGMP(Cyclic Guanosine Monophosphate) ELISA Kit
Quanticon™ cGMP(Cyclic Guanosine Monophosphate) ELISA Kit
Quanticon™ cGMP(Cyclic Guanosine Monophosphate) ELISA Kit
Principle of the Procedure
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with cGMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to cGMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cGMP in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name | Quanticon™ cGMP(Cyclic Guanosine Monophosphate) ELISA Kit |
SKU | ABQK-EC96-cGMPge |
Applications | ELISA |
Reactivity | General |
Alternative Name | c-GMP, 3′,5′-Cyclic GMP, Cyclic 3′,5′-GMP, Guanosine 3′,5′-Cyclic Phosphate |
Standard | 10000 pg/mL |
Sensitivity | 43.8 pg/mL |
Detection Range | 156.25-10000 pg/mL |
Sample Type | Serum, Plasma, Tissue homogenate, Urine, Saliva, Cell culture supernatant and Other biological samples |
Sample Volume | 50 ÎĽL |
Assay Type | Competitive Inhibition |
Assay Duration | 1.5 H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
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