CheKine™ Micro Coenzyme Ⅰ NAD(H) Assay Kit
CheKine™ Micro Coenzyme Ⅰ NAD(H) Assay Kit
CheKine™ Micro Coenzyme Ⅰ NAD(H) Assay Kit
Applications Notes
Abbkine CheKine™ Micro Coenzyme Ⅰ NAD(H) Assay Kitprovides a convenient tool for sensitive detection of the NAD and NADH, and their ratio in various tissues and subcellular organelles. This Kit is based on enzyme cycling reaction (It does not recognize NADP+/NADPH), in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at OD450 nm, is proportionate to the NAD+ /NADH concentration in the sample.
Product Name | CheKine™ Micro Coenzyme Ⅰ NAD(H) Assay Kit |
Catalogue No | KTB1020 |
Kit components | • Assay Buffer • EtOH Solution • WST-8 Solution • Enhancer • NAD Cycling Enzyme Mix • NAD standard (10 mM) • NAD Extraction Buffer • NADH Extraction Buffer |
Calibration range | 0.78 µM-50 µM |
Features & Benefits | • Detect NAD+/NADH concentrations and ratios in serum, plasma，cell or plant/animal tissue extracts. • Detection limit of 0.78 µM and linearity up to 50 µM NAD+/NADH in 96-well plate assay. |
Storage | Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
Shipping | Gel pack with blue ice. |
Formulations | Liquid solution ready to use |
Background | Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP-ribosylation reactions and as a substrate for sirtuins. |
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