Product Name

CheKine™ Micro Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Catalogue No

KTB1640

Applications Notes

GSH-Px catalyzes H2O2 to oxidize GSH to produce GSSG; glutathione reductase (GR) catalyzes NADPH to reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+; NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not; NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GSH-Px activity.

Kit components

• Assay Buffer

• Substrate

• GR

• H2O2

Features & Benefits 

• Determination of GSH-Px activity in serum, plasma, plant tissue/cell lysates and other biological fluids.

Storage

Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Shipping

Gel pack with blue ice.

Formulations

Liquid solution ready to use

Background

GSH-Px is one of the main enzymes that catalyze the oxidation of reduced glutathione (GSH) in the glutathione redox cycle. GSH-Px not only specifically catalyzes the reaction between reduced glutathione and ROS to form oxidized glutathione GSSG, thereby protecting the biofilm from ROS damage and maintaining the normal function of cells; it also protects the liver and improves the body immunity, antagonizing the harmful effects of harmful metal ions on the body and increasing the body’s ability to resist radiation.