Quanticon™ 5-HT(5-Hydroxytryptamine) ELISA Kit
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 5-HT. During the reaction, 5-HT in the sample or standard competes with a fixed amount of 5-HT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 5-HT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 5-HT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Product Name | Quanticon™ 5-HT(5-Hydroxytryptamine) ELISA Kit |
SKU | ABQ-EC96-5HTge |
Applications | ELISA |
Reactivity | General |
Alternative Name | ST, 5-HT , Serotonin , 5-Hydroxytryptamine |
Standard | 900 ng/mL |
Sensitivity | 3.97 ng/mL. |
Detection Range | 14.07-900 ng/mL |
Sample Type | Serum, Plasma, Tissue homogenate and Other biological samples |
Sample Volume | 50 ÎĽL |
Assay Type | Competitive |
Assay Duration | 1.5H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
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