Quanticon™ Chloramphenicol (CAP) ELISA Kit

Principle of the Procedure        

This assay employs the competitive inhibition enzyme immunoassay technique. The kit consists of an enzyme-labeled plate pre-coated with coupled antigens, horseradish enzyme markers, antibodies, standard substances, and other supporting reagents. During detection, standard substances or sample solutions are added. Chloramphenicol in the samples competes with the pre-coated coupled antigens on the enzyme-labeled plate for Chloramphenicol specific antibody. After adding the enzyme markers, TMB substrate is used for color development. The absorbance value of the sample is negatively correlated with the content of Chloramphenicol it contains, and the residual amount of Chloramphenicol in the sample can be obtained by comparing with the standard curve.