Quanticon™ Glutathione Reductase Kit

Quanticon™ Glutathione Reductase Kit

GLUTATHIONE REDUCTASE (GR) reduces oxidized glutathione (GSSG) to the reduced sulfhydryl form GSH which is an important cellular antioxidant. A high GSH/GSSG ratio is important for protection against oxidative stress. Thus, measurement of GR activity is used as indicator for oxidative stress. Amplicon Biotech non- radioactive, colorimetric GR assay is designed to accurately measure GR activity in biological samples with a method that utilizes Ellman’s method in which DTNB reacts with the GSH generated from the reduction of GSSG by the GR in a sample to form a yellow product (TNB2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to GR activity in the sample.

Cat no: ABQ-BC100-GRA
ABQ-BC100-GRA
Size: Rate
Rate
100T: $520
$520

Product name

Quanticon™ Glutathione Reductase Kit

SKU

ABQ-BC100-GRA

Applications

ASSAY

Reactivity

General  

Detection Range

0.6 – 100 ÎĽM in 96-well plate

Sample Type

serum, plasma, foods and other biological fluids

Sample Volume

100 ÎĽL

Assay Type

Competitive

Assay Duration

40min

Detection Wavelength

412nm

Storage

2-8oC/-20 oC

 

Citations

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Quanticon™ Glutathione Reductase Kit

Quanticon™ Glutathione Reductase Kit

Quanticon™ Glutathione Reductase Kit

assay kit

GLUTATHIONE REDUCTASE (GR) reduces oxidized glutathione (GSSG) to the reduced sulfhydryl form GSH which is an important cellular antioxidant. A high GSH/GSSG ratio is important for protection against oxidative stress. Thus, measurement of GR activity is used as indicator for oxidative stress. Amplicon Biotech non- radioactive, colorimetric GR assay is designed to accurately measure GR activity in biological samples with a method that utilizes Ellman’s method in which DTNB reacts with the GSH generated from the reduction of GSSG by the GR in a sample to form a yellow product (TNB2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to GR activity in the sample.

Cat no: ABQ-BC100-GRA ABQ-BC100-GRA Size: Rate Rate 100T: $520 $520 Datasheet Get Quote MS/DS COA

Product name

Quanticon™ Glutathione Reductase Kit

SKU

ABQ-BC100-GRA

Applications

ASSAY

Reactivity

General  

Detection Range

0.6 – 100 ÎĽM in 96-well plate

Sample Type

serum, plasma, foods and other biological fluids

Sample Volume

100 ÎĽL

Assay Type

Competitive

Assay Duration

40min

Detection Wavelength

412nm

Storage

2-8oC/-20 oC

 

Citations

Product name

Quanticon™ Glutathione Reductase Kit

SKU

ABQ-BC100-GRA

Applications

ASSAY

Reactivity

General  

Detection Range

0.6 – 100 ÎĽM in 96-well plate

Sample Type

serum, plasma, foods and other biological fluids

Sample Volume

100 ÎĽL

Assay Type

Competitive

Assay Duration

40min

Detection Wavelength

412nm

Storage

2-8oC/-20 oC

 

Citations

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Principle of the Procedure

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig CCK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig CCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig CCK in the samples is then determined by comparing the OD of the samples to the standard curve.

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