Quanticon™ Catalase Assay Kit

Quanticon™ Catalase Assay Kit

Catalase, is a ubiquitous antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (Hâ‚‚Oâ‚‚ ) to water and oxygen.

2H₂O₂ → 2H₂O + O₂

By preventing excessive H₂O₂ build up, catalase allows important cellular processes which produce H₂O₂ as a byproduct to occur safely. Deficiency in catalase activity has been associated with grey hair and peroxisomal disorder acatalasia. Simple, direct and high-throughput assays for catalase activity find wide applications. Amplicon Biotech improved assay directly measures catalase degradation of H₂O₂ using a redox dye. The change in color intensity at 570nm or fluorescence intensity (λem/ex = 585/530nm) is directly proportional to the catalase activity in the sample.

Cat no: ABQ-BC100-CAA
ABQ-BC100-CAA
Size: Rate
Rate
100T: $520
$520

Product name

Quanticon™ Catalase Assay Kit

SKU

ABQ-BC100-CAA

Applications

ASSAY

Reactivity

General  

Detection Range

0.2 – 5 U/L (OD) or 0.02 – 0.5 U/L

Detection Limit

0.2 U/L

Sample Type

serum, plasma, foods and other biological fluids

Sample Volume

100 ÎĽL

Assay Type

Competitive

Assay Duration

40min

Detection Wavelength

570nm

Storage

2-8oC/-20 oC

 

Citations

Our latest product

Quanticon™ Catalase Assay Kit

Quanticon™ Catalase Assay Kit

Quanticon™ Catalase Assay Kit

assay kit

Catalase, is a ubiquitous antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (Hâ‚‚Oâ‚‚ ) to water and oxygen.

2H₂O₂ → 2H₂O + O₂

By preventing excessive H₂O₂ build up, catalase allows important cellular processes which produce H₂O₂ as a byproduct to occur safely. Deficiency in catalase activity has been associated with grey hair and peroxisomal disorder acatalasia. Simple, direct and high-throughput assays for catalase activity find wide applications. Amplicon Biotech improved assay directly measures catalase degradation of H₂O₂ using a redox dye. The change in color intensity at 570nm or fluorescence intensity (λem/ex = 585/530nm) is directly proportional to the catalase activity in the sample.

Cat no: ABQ-BC100-CAA ABQ-BC100-CAA Size: Rate Rate 100T: $520 $520 Datasheet Get Quote MS/DS COA

Product name

Quanticon™ Catalase Assay Kit

SKU

ABQ-BC100-CAA

Applications

ASSAY

Reactivity

General  

Detection Range

0.2 – 5 U/L (OD) or 0.02 – 0.5 U/L

Detection Limit

0.2 U/L

Sample Type

serum, plasma, foods and other biological fluids

Sample Volume

100 ÎĽL

Assay Type

Competitive

Assay Duration

40min

Detection Wavelength

570nm

Storage

2-8oC/-20 oC

 

Citations

Product name

Quanticon™ Catalase Assay Kit

SKU

ABQ-BC100-CAA

Applications

ASSAY

Reactivity

General  

Detection Range

0.2 – 5 U/L (OD) or 0.02 – 0.5 U/L

Detection Limit

0.2 U/L

Sample Type

serum, plasma, foods and other biological fluids

Sample Volume

100 ÎĽL

Assay Type

Competitive

Assay Duration

40min

Detection Wavelength

570nm

Storage

2-8oC/-20 oC

 

Citations

Our latest product

Principle of the Procedure

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig CCK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig CCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig CCK in the samples is then determined by comparing the OD of the samples to the standard curve.

Citations  Â