Quanticon™ Human ADAMTS5(A Disintegrin And Metalloproteinase With Thrombospondin 5) ELISA Kit
Principle of the Procedure
This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Human ADAMTS5. Standards or samples containing Human ADAMTS5 are added to the plate and reacted with capture antibody. A second anti-Human ADAMTS5 antibody labeled biotin is then added and binds to Human ADAMTS5 captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Human ADAMTS5-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Human ADAMTS5 in the samples is then calculated from the OD value by establishing a standard curve.
Product Name | Quanticon™ Human ADAMTS5(A Disintegrin And Metalloproteinase With Thrombospondin 5) ELISA Kit |
SKU | ABQ-ES96-ADAMTS5hu |
Applications | ELISA |
Reactivity | Human |
Alternative Name | ADAMTS5, ADAM-TS 11, ADAM-TS 5, ADAM-TS5, ADAMTS-11, ADAMTS-5, ADAMTS11, ADMP-2 |
Standard | 50ng/mL |
Sensitivity | 0.47 ng/mL |
Detection Range | 0.79-50ng/mL |
Sample Type | Serum, Plasma, Tissue homogenate and Other biological samples |
Sample Volume | 100 ÎĽL |
Assay Type | Sandwich |
Assay Duration | 3.5 H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
Â
Citations
