Quanticon™ Human AMBP(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit
Principle of the Procedure
This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Human AMBP. Standards or samples containing Human AMBP are added to the plate and reacted with capture antibody. A second anti-Human AMBP antibody labeled biotin is then added and binds to Human AMBP captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Human AMBP-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Human AMBP in the samples is then calculated from the OD value by establishing a standard curve.
Product Name | Quanticon™ Human AMBP(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit |
SKU | ABQ-ES96-AMBPhu |
Applications | ELISA |
Reactivity | Human |
Alternative Name | Protein AMBP, alpha-1-microglobulin (A1M), Protein HC, Bikunin |
Standard | 300ng/mL. |
Sensitivity | 2.81 ng/mL. |
Detection Range | 4.69-300ng/mL. |
Sample Type | Serum, Plasma, Tissue homogenate and Other biological samples |
Sample Volume | 100 ÎĽL |
Assay Type | Sandwich |
Assay Duration | 3.5H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
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