Quanticon™ Human CASP7(Caspase 7) ELISA Kit
Principle of the Procedure
This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Human CASP7. Standards or samples containing Human CASP7 are added to the plate and reacted with capture antibody. A second anti-Human CASP7 antibody labeled biotin is then added and binds to Human CASP7 captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Human CASP7-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Human CASP7 in the samples is then calculated from the OD value by establishing a standard curve.
Product Name | Quanticon™ Human CASP7(Caspase 7) ELISA Kit |
SKU | ABQ-ES96-CASP7hu |
Applications | ELISA |
Reactivity | Human |
Alternative Name | CASP7, CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3, Caspase 7 |
Standard | 20ng/mL |
Sensitivity | 0.19 ng/mL |
Detection Range | 0.32-20ng/mL. |
Sample Type | Serum, Plasma, Tissue homogenate Cell culture supernatant and Other biological samples |
Sample Volume | 100 ÎĽL |
Assay Type | Sandwich |
Assay Duration | 3.5 H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
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