Quanticon™ Rat α-Glu(Alpha Glucosidase) ELISA Kit
Principle of the Procedure
This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Rat α-Glu. Standards or samples containing Rat α-Glu are added to the plate and reacted with capture antibody. A second anti-Rat α-Glu antibody labeled biotin is then added and binds to Rat α-Glu captured on the plate. After that, Streptavidin-Horseradish Peroxidase(SA-HRP) is added to form a sandwich complex of solid phase antibody-Rat α-Glu-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Rat α-Glu in the samples is then calculated from the OD value by establishing a standard curve.
Product Name | Quanticon™ Rat α-Glu(Alpha Glucosidase) ELISA Kit |
SKU | ABQ-ES96-AGLUrt |
Applications | ELISA |
Reactivity | Rat |
Alternative Name | α-Glu |
Standard | 100ng/mL |
Sensitivity | 0.94 ng/mL. |
Detection Range | 1.57-100ng/mL. |
Sample Type | Serum, Plasma, Tissue homogenate and Other biological samples |
Sample Volume | 100 μL |
Assay Type | Sandwich |
Assay Duration | 3.5 H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
Citations
