Quanticon™ Rat FGF21(Fibroblast Growth Factor 21) ELISA Kit
This kit uses the Sandwich-ELISA principle. The microtiter plate strips has been pre-coated with an affinity purified antibody to Rat FGF21. Standards or samples containing Rat FGF21 are added to the plate and reacted with capture antibody. A second anti-Rat FGF21 antibody labeled biotin is then added and binds to Rat FGF21 captured on the plate. After that, Streptavidin-Horseradish Peroxidase (SA-HRP) is added to form a sandwich complex of solid phase antibody-Rat FGF21-biotin labeled antibody-SA-HRP. And then, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Rat FGF21 in the samples is then calculated from the OD value by establishing a standard curve.
Product Name  | Quanticon™ Rat FGF21(Fibroblast Growth Factor 21) ELISA Kit |
SKU | ABQ-ES96-FGF21rt |
Applications | ELISA |
Reactivity | Rat |
Alternative Name | FGF-21 |
Standard | 2000pg/mL. |
Sensitivity | 18.75 pg/mL. |
Detection Range | 31.25-2000pg/mL. |
Sample Type | Serum, Plasma, Tissue homogenate Cell culture supernatant and Other biological samples |
Sample Volume | 100 ÎĽL |
Assay Type | Sandwich |
Assay Duration | 3.5 H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
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