Quanticon™ Rat E2(Estradiol) ELISA Kit
Principle of the Procedure
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat E2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name | Quanticon™ Rat E2(Estradiol) ELISA Kit |
SKU | ABQK-EC96-E2rt |
Applications | ELISA |
Reactivity | Rat |
Alternative Name | Estradiol , E2 |
Standard | 3000 pg/mL |
Sensitivity | 14.45 pg/mL |
Detection Range | 46.88-3000 pg/mL |
Sample Type | Serum, Plasma, Tissue homogenate, Urine, Saliva, and Cell culture supernatants and other biological fluids |
Sample Volume | 50 ÎĽL |
Assay Type | Competitive |
Assay Duration | 2.5 H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
Citations Â
