Quanticon™ Rat VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit
Principle of the Procedure
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VEGFA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VEGFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VEGFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name | Quanticon™ Rat VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit |
SKU | ABQK-ES96-VEGFArt |
Applications | ELISA |
Reactivity | Rat |
Alternative Name | VEGF, VEGF-A, VPF, Vascular Endothelial Cell Growth Factor A |
Standard | 1000 pg/mL |
Sensitivity | 6.3 pg/mL |
Detection Range | 15.63-1000 pg/mL |
Sample Type | Serum, Plasma, Tissue homogenate, Urine, Saliva, Cerebrospinal fluid (CSF), Cell culture supernatant and Other biological samples |
Sample Volume | 100 ÎĽL |
Assay Type | Sandwich |
Assay Duration | 3.5 H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
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