Quanticon™ TXA2(Thromboxane A2) ELISA Kit

Quanticon™ TXA2(Thromboxane A2) ELISA Kit

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with TXA2. During the reaction, TXA2 in the sample or standard competes with a fixed amount of TXA2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to TXA2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TXA2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Cat no: ABQ-EC96-TXA2ge
ABQ-EC96-TXA2ge
Size: Rate
Rate
96T: $450
$450
48T: $230
$230

Product Name

Quanticon™ TXA2(Thromboxane A2) ELISA Kit

SKU

ABQ-EC96-TXA2ge

Applications

ELISA

Reactivity

General/ Universal

Alternative Name

TXA2

Standard

1000 pg/mL.

Sensitivity

4.39 pg/mL.

Detection Range

15.63-1000 pg/mL.

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples

Sample Volume

50 ÎĽL

Assay Type

Competitive

Assay Duration

1.5H

Detection Wavelength

450nm

Storage

2-8oC/-20 oC

 

Citations   

Quanticon™ TXA2(Thromboxane A2) ELISA Kit

Quanticon™ TXA2(Thromboxane A2) ELISA Kit

Quanticon™ TXA2(Thromboxane A2) ELISA Kit

elisa-kit-images-e1737629308569-removebg-preview
Untitled design (1)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with TXA2. During the reaction, TXA2 in the sample or standard competes with a fixed amount of TXA2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to TXA2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TXA2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Cat no: ABQ-EC96-TXA2ge ABQ-EC96-TXA2ge Size: Rate Rate 96T: $450 $450 48T: $230 $230 Datasheet Get Quote MS/DS COA

Product Name

Quanticon™ TXA2(Thromboxane A2) ELISA Kit

SKU

ABQ-EC96-TXA2ge

Applications

ELISA

Reactivity

General/ Universal

Alternative Name

TXA2

Standard

1000 pg/mL.

Sensitivity

4.39 pg/mL.

Detection Range

15.63-1000 pg/mL.

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples

Sample Volume

50 ÎĽL

Assay Type

Competitive

Assay Duration

1.5H

Detection Wavelength

450nm

Storage

2-8oC/-20 oC

 

Citations   

Product Name

Quanticon™ TXA2(Thromboxane A2) ELISA Kit

SKU

ABQ-EC96-TXA2ge

Applications

ELISA

Reactivity

General/ Universal

Alternative Name

TXA2

Standard

1000 pg/mL.

Sensitivity

4.39 pg/mL.

Detection Range

15.63-1000 pg/mL.

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples

Sample Volume

50 ÎĽL

Assay Type

Competitive

Assay Duration

1.5H

Detection Wavelength

450nm

Storage

2-8oC/-20 oC

 

Citations   

Principle of the Procedure

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig CCK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig CCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig CCK in the samples is then determined by comparing the OD of the samples to the standard curve.

Citations  Â