Quanticon™ XcelStep Human PGI(Pepsinogen I) ELISA Kit
Quanticon™ XcelStep Human PGI(Pepsinogen I) ELISA Kit
Principle of the Procedure
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PGI, and the Human PGI standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PGI . After TMB substrate solution is added, only those wells that contain Human PGI and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGI in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name | Quanticon™ XcelStep Human PGI(Pepsinogen I) ELISA Kit |
SKU | ABQK-ZS96-PGIhu |
Applications | ELISA |
Reactivity | Human |
Alternative Name | Pg5, PGA5, PGA, PG-A, Pepsinogen 5 Group I |
Standard | 20 ng/mL |
Sensitivity | 18 pg/mL |
Detection Range | 0.32-20 ng/mL |
Sample Type | Serum, Plasma, Tissue homogenate, Urine, Saliva, Cerebrospinal fluid (CSF), Cell culture supernatant and Other biological samples |
Sample Volume | 50 ÎĽL |
Assay Type | Sandwich |
Assay Duration | 1.5 H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
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