Quanticon™ Rat S100B(S100 Calcium Binding Protein B) ELISA Kit
Principle of the Procedure
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat S100B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat S100B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat S100B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat S100B in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name | Quanticon™ Rat S100B(S100 Calcium Binding Protein B) ELISA Kit |
SKU | ABQK-ES96-S100Brt |
Applications | ELISA |
Reactivity | Rat |
Alternative Name | S100-B, NEF, S100Beta, S-100 protein subunit beta |
Standard | 2000pg/mL. |
Sensitivity | 13.1pg/mL |
Detection Range | 31.2-2000pg/mL. |
Sample Type | Serum, Plasma, Tissue homogenate, Urine, Saliva, Cerebrospinal fluid (CSF), Cell culture supernatant and Other biological samples |
Sample Volume | 100 ÎĽL |
Assay Type | Sandwich |
Assay Duration | 3.5 H |
Detection Wavelength | 450nm |
Storage | 2-8oC/-20 oC |
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